SummaryNine Hypericum species (H. barbatum, H. hirsutum, H. linarioides, H. olympicum, H. perforatum, H. rochelii, H. rumeliacum, H. tetrapterum and H. umbellatum) collected in Serbia were assayed for inhibitory potential against xanthine oxidase in vitro, on the commercial enzyme, and compared with allopurinol. Seven studied Hypericum species (H. barbatum, H. rochelii, H. rumeliacum, H. umbellatum, H. perforatum, H. tetrapterum and H. olympicum) inhibit commercial xanthine oxidase with an IC50 below 100 μg/mL. H. barbatum exerted the most potent inhibitory effect (IC50 = 31.84 ± 6.64 μg/mL), followed closely by H. perforatum (IC50 = 37.12 ± 4.06 μg/mL).
References
1.
Spiteller M, Özen T, Smelcerovic A, Zuehlke S, Mimica-Dukić N. Phenolic constituents and the in vitro antioxidant activity of the flowers of Hypericum venustum. Fitoterapia. 2008;79(3):191–3.
2.
Radi R, Rubbo H, Bush K, Freeman BA. Xanthine Oxidase Binding to Glycosaminoglycans: Kinetics and Superoxide Dismutase Interactions of Immobilized Xanthine Oxidase–Heparin Complexes. Archives of Biochemistry and Biophysics. 1997;339(1):125–35.
3.
Smelcerovic A, Verma V, Spiteller M, Ahmad SM, Puri SC, Qazi GN. Phytochemical analysis and genetic characterization of six Hypericum species from Serbia. Phytochemistry. 2006;67(2):171–7.
4.
Radulović N, Stankov-Jovanović V, Stojanović G, Šmelcerović A, Spiteller M, Asakawa Y. Screening of in vitro antimicrobial and antioxidant activity of nine Hypericum species from the Balkans. Food Chemistry. 2007;103(1):15–21.
5.
Benedı́ J, Arroyo R, Romero C, Martı́n-Aragón S, Villar AM. Antioxidant properties and protective effects of a standardized extract of Hypericum perforatum on hydrogen peroxide-induced oxidative damage in PC12 cells. Life Sciences. 2004;75(10):1263–76.
6.
Bonkanka C, Smelcerovic A, Zuehlke S, Rabanal R, Spiteller M, Sánchez-Mateo C. HPLC-MS Analysis and Anti-Oedematogenic Activity ofHypericum grandifoliumChoisy (Hypericaceae). Planta Medica. 2008;74(07):719–25.
7.
Hufsky F, Scheubert K, Böcker S. New kids on the block: novel informatics methods for natural product discovery. Natural Product Reports. 2014;31(6):807.
8.
Verma V, Smelcerovic A, Zuehlke S, Hussain MA, Ahmad SM, Ziebach T, et al. Phenolic constituents and genetic profile of Hypericum perforatum L. from India. Biochemical Systematics and Ecology. 2008;36(3):201–6.
9.
Lü JM, Yao Q, Chen C. 3,4-Dihydroxy-5-nitrobenzaldehyde (DHNB) is a potent inhibitor of xanthine oxidase: A potential therapeutic agent for treatment of hyperuricemia and gout. Biochemical Pharmacology. 2013;86(9):1328–37.
10.
Stojanovic G, Dordevic A, Smelcerovic A. Do Other Hypericum Species Have Medical Potential As St. John’s Wort (Hypericum perforatum)? Current Medicinal Chemistry. 2013;20(18):2273–95.
11.
Havlik J, de la Huebra RG, Hejtmankova K, Fernandez J, Simonova J, Melich M, et al. Xanthine oxidase inhibitory properties of Czech medicinal plants. Journal of Ethnopharmacology. 2010;132(2):461–5.
The statements, opinions and data contained in the journal are solely those of the individual authors and contributors and not of the publisher and the editor(s). We stay neutral with regard to jurisdictional claims in published maps and institutional affiliations.
