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AFMN Biomedicine is an international, peer-reviewed, open-access journal dedicated to publishing high-quality original research and review articles in basic, translational, and clinical biomedicine, emphasizing discoveries of broad significance for the health sciences.

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Vol. 42, No. 4 (2025):

Published: 17.12.2025.

AFMN BIOMEDICINE

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15.03.2026. Original article Online first
EXPRESSION OF ANOCTAMIN-1 IN HUMAN GASTROINTESTINAL TRACT DURING EMBRYONIC AND FETAL DEVELOPMENT

By Vladimir Petrovic, Aleksandra Veličkov, Marko Jović, Julija Radenković, Braca Kundalić, Dušan Miljković, Vukota Radovanović, Goran Radenković

Anoctamin 1 (ANO1, TMEM16A) is a transmembrane protein belonging to the ANO family, with a role in the formation of calcium-activated chloride channels (CaCC). It is included in the regulation of physiological processes such as muscle contraction, gastrointestinal motility, secretion, and electrical excitability. Also, recent data suggest that ANO1 is a specific marker for interstitial cells of Cajal (ICC). The aim of the paper was to examine the spatial and temporal distribution of ANO1 in the human stomach, small intestine, and large intestine during embryofetal development as a potential marker for the differentiation of ICC and smooth muscle cells. As a material, we used 2 embryos and samples from 21 fetuses. The tissue samples were routinely processed into paraffin blocks, and 5 µm-thick sections were immunostained for ANO1. Our results show that ANO1 appears during embryonic development (8th week), and its expression continues through the fetal stages. Epithelial, endothelial and ICC cells consistently expressed ANO1 in all examined samples. Smooth muscle cells showed strong expression in muscularis propria, however by the 25th week this immunopositivity was absent from outer muscle layers in stomach and large intestine. In conclusion, ANO1 can be considered as a reliable marker for following the differentiation of SMC and ICC during embryonic and fetal development.

15.03.2026. Review article Online first
THE ROLE OF PROINFLAMMATORY CYTOKINES IL-6 AND IL-17A AND ANTI-INFLAMMATORY CYTOKINE IL-10 IN COLORECTAL CANCER

By Lana Sarajlić, Edin Hodžić, Alma Mekić Abazović, Samir Muhović, Amina Mehić, Emsad Halilović, Salem Bajramagić, Adnan Kulo, Ramajana Šukić-Karalić

Colorectal cancer (CRC) remains a leading cause of cancer-related mortality worldwide, with chronic inflammation recognized as a critical factor in its pathogenesis. This review focuses on the roles of pro-inflammatory cytokines interleukin-6 (IL-6) and interleukin-17A (IL-17A), alongside the anti-inflammatory cytokine interleukin-10 (IL-10), in CRC development and progression. The reason we chose theese cytokines amoung others is that we found certain number of similar research in recent literature for comparision, condidering this topic is quite rare. Elevated levels of IL-6 and IL-17A have been linked to enhanced tumor proliferation, survival, invasion, and metastasis, highlighting their contribution to a tumor-promoting microenvironment. Conversely, IL-10 exhibits a dual role by suppressing inflammation yet potentially facilitating immune evasion and tumor progression in certain contexts. Understanding the complex interplay and signaling pathways of these cytokines may improve CRC risk assessment, diagnosis, prognosis, and offer new avenues for targeted therapies. This review synthesizes current evidence from recent literature to elucidate the molecular mechanisms and clinical implications of IL-6, IL-17A, and IL-10 in colorectal cancer.

15.03.2026. Original article Online first
MONITORING MICROCIRCULATION IN THE PALATAL MUCOSA BENEATH AN UPPER COMPLETE DENTURE USING A LASER DOPPLER FLOWMETRY: A PRELIMINARY STUDY

By Petar Tekić, Stefan Vulović, Marija Milić, Marko Igić, Milena Kostić, Aleksandra Popovac, Ivica Stančić, Aleksandra Milić Lemić

Older edentulous adults often rely on mucosa-supported complete dentures (CDs) due to limitations that preclude implant therapy. Although an adequate retention of maxillary CD is essential for functional efficiency, compression of the palatal mucosa during impression making may compromise palatal mucosal microcirculation during denture wear. The aim was to evaluate short-term changes in palatal mucosal blood perfusion in elderly individuals during adaptation to newly fabricated maxillary CDs. Ten fully edentulous participants (mean age 67.3 years) received conventionally fabricated maxillary CDs and custom-made thermoplastic splints replicating the denture base. Palatal microcirculation was measured using laser Doppler flowmetry (LDF) through perforations in the splints at three regions (frontal, premolar, and molar), and at four time points: before denture insertion (T0), and at 30 min (T1), one week (T2), and six weeks (T3) post-insertion. Significant reductions in blood perfusion units (BPU) were observed over time in the premolar and molar regions over time (p < 0.05), while changes in the frontal region were not significant (p > 0.05). At each time point, the molar region exhibited higher BPU values than the other regions. The greatest reductions were noted between the initial (T0/T1) and later (T2/T3) stages. Short-term use of maxillary CDs was associated with reduced palatal mucosal blood perfusion, particularly in posterior regions. These findings underscore the importance of monitoring tissue response during maxillary complete denture adaptation to improve therapy outcomes in elderly patients.

15.03.2026. Original article Online first
RELIABILITY OF ELISA TEST IN THERAPEUTIC DRUG MONITORING OF ADALIMUMAB AND INFLIXIMAB IN PEDIATRIC INFLAMMATORY BOWEL DISEASE

By Jasmina Katanić, Dejan Dobrijević, Mirjana Stojšić

15.03.2026. Case report Online first
18F-FLUORODEOXYGLUCOSE POSITRON EMISSION TOMOGRAPHY WITH COMPUTED TOMOGRAPHY IN THE DIAGNOSIS OF LOEFFLER'S ENDOCARDITIS: A CASE REPORT

By Milica Kotur, Nikola Pantić, Lenka Grujičić, Isidora Grozdić-Milojević, Dragana Šobić-Šaranović, Vera Artiko, Strahinja Odalović

15.03.2026. Original article Online first
SUSTAINABILITY OF HEALTHCARE SYSTEMS: INTEGRATION OF GREEN TECHNOLOGY IN HOSPITAL FACILITIES

By Marko Kimi Milić, Šćepan Sinanović, Tanja Prodović, Saša Bubanj

Current issue
17.12.2025. Review article
ANTI-INFLAMMATORY EFFECT OF BILBERRY (VACCINIUM MYRTILLUS L.) PREPARATION: A SYSTEMATIC REVIEW

By Anđela V. Dragićević, Nikola M. Stojanović, Dragana R. Pavlović

17.12.2025. Review article
EPILEPSY IN CHILDREN WITH CENTRAL NERVOUS SYSTEM INFECTIONS: A SYSTEMATIC REVIEW AND META-ANALYSIS

By Mahsa Afrand, Niusha Rostampur, Zeinab Pourhadi, Javad Rezanezhad, Pouriya Nekoueifard

17.12.2025. Original article
EFFECT OF A SUBANESTHETIC DOSE OF KETAMINE ON AWAKENING AND POSTOPERATIVE DEPRESSION AND ANXIETY IN PATIENTS UNDERGOING CORONARY ARTERY BYPASS GRAFT SURGERY

By Rasool Ferasatkish, Nahid Aghdaii, Ali Sadeghi, Mohsen Ziyaeifard, Rasoul Azarfarin, Mirahmad Hendinezhad, Anahita Babaei

17.12.2025. Original article
PROTECTIVE EFFECTS OF GREEN TEA AND BILBERRY AGAINST SECONDARY HYPERLIPIDEMIA IN GENTAMICIN‒INDUCED NEPHROTOXICITY IN RATS

By Milica Veljković, Nikola M. Stojanović, Tanja Džopalić, Dragana R. Pavlović, Dušan Sokolović, Milan Petković

Recent issues
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31.10.2025. Review article
ACUTE EFFECTS OF VARIOUS EXERCISE MODALITIES ON GLYCEMIC CONTROL IN PATIENTS WITH TYPE 2 DIABETES: A SYSTEMATIC REVIEW

By Anja Lazić, Tatjana Jevtovic Stoimenov, Nebojša Trajković

Type 2 diabetes mellitus (T2DM) is characterized by impaired glycemic control, which increases the risk of cardiovascular and metabolic complications. Exercise is a key non-pharmacological intervention known to improve blood glucose regulation, but the acute effects of different exercise modalities on glycemic control in T2DM remain unclear. The aim of this systematic review was to critically analyze and synthesize the existing body of research on the acute effects of various exercise modalities on glycemic control in patients with T2DM. This systematic review included studies involving adults (≥ 18 years) with T2DM where structured exercise program is the primary or significant intervention, assessing outcomes related to glycemic control (HbA1c, and fasting glucose). A comprehensive search was conducted across two electronic databases (Web of Science and PubMed) using structured search terms like "acute", "exercise", "type 2 diabetes", and "glycemic control". Study selection involved two independent reviewers screening articles, with disagreements resolved through discussion or third-party consultation, followed by detailed data extraction on study characteristics, intervention details, and outcomes. Ten studies were identified that met all inclusion criteria. This systematic review highlights that moderate intensity continuous training and high-intensity interval training have positive acute effects on glycemic control in individuals with T2DM. These findings suggest that both modalities are effective non-pharmacological strategies for optimizing glycemic control in patients T2DM.

01.12.2021. Original article
Effect of zinc supplement on sleep quality in older adults: A randomized clinical trial study

By Abolfazl Afzali, Mojarrad Mohandes, Shahrbanoo Goli, Hossein Bagheri, Seyedmohammad Mirhosseini, Hossein Ebrahimi

01.12.2023. Review article
Pharmacokinetics of herb-drug interactions: Experimental models in Nigeria

By Mary Ologe, Yusuf Olalekan, Awodayo Adepiti, Olubunmi Atolani, Anoka Njan, Adedibu Tella, Ezekiel Iwalewa

01.12.2024. Original article
The application of artificial intelligence in the healthcare system management in the Republic of Serbia: Enhancing efficiency, predictive capacity, and decision-making

By Milić Kimi, Šćepan Sinanović, Tatjana Kilibarda, Saša Bubanj, Novica Bojanić, Tanja Prodović

31.10.2025. Review article
EMERGENCY CONDITIONS IN PARKINSON’S DISEASE

By Jelena Stamenović, Vuk Milošević, Vanja Đurić

Parkinson's disease (PD) is a chronic, neurodegenerative disorder that in certain stages can present a series of acute symptoms and signs, the development of which lasts several hours or days. Emergencies in PD can be a direct consequence of the pathophysiology of the disease or a secondary consequence of the administration of antiparkinsonian drugs. Urgent conditions in PD can also occur due to falls, infectious diseases, after deep brain stimulation or surgical treatment of other accompanying diseases.This article describes the following emergency conditions: parkinsonism-hyperpyrexia syndrome, dyskinesia hyperpyrexia syndrome, occurrence of acute psychosis and delirium during PD. Morbidity and mortality in these disorders are a consequence of the inability to make a timely diagnosis and provide appropriate therapeutic treatment. Timely diagnosis and treatment are very important for reducing the mortality and morbidity rates.

31.10.2025. Original article
INVESTIGATING FACTORS INFLUENCING CLINICAL PREGNANCY RATES IN HORMONE REPLACEMENT THERAPY FROZEN-THAWED EMBRYO TRANSFER CYCLES: A CROSS-SECTIONAL STUDY

By Sepideh Peivandi, Samaneh Aghajanpour, Mohammad Khademloo, Keshvar Samadaee Gelehkolaee, Marzieh Zamaniyan

31.10.2025. Review article
BIOLOGICAL PROPERTIES OF BUILDING DENTAL MATERIALS AND CLINICAL CHANGES IN ORAL TISSUES CAUSED BY THEIR APPLICATION: A NARRATIVE REVIEW

By Ana Pejčić, Milena Kostić, Ivana Stankovic, Radmila Obradovic, Marija Bradic-Vasic, Marija Đorđević, Marko Igic, Nikola Gligorijević

Restoring the morphological and functional integrity of damaged or lost teeth and replacing them with suitable materials remains a significant challenge in modern dentistry. A continuous development of new restorative materials aims to improve mechanical properties, aesthetic outcomes, and longevity of dental restorations, while minimizing adverse biological effects. Biocompatibility represents a fundamental requirement of all dental materials, referring to their ability to perform a specific function in the oral environment without eliciting undesirable local or systemic tissue responses. The oral mucosa, including the lips, is constantly exposed to numerous physical, chemical, and biological agents that may act as irritants or sensitizers. Given that most dental materials are designed for prolonged intraoral use, their continuous contact with oral tissues can influence mucosal integrity and function. Clinical manifestations of adverse reactions vary in severity and presentation, often depending on the material composition, exposure duration, and individual patient sensitivity. Local tissue reactions associated with dental materials include conditions such as oral stomatitis, mechanical trauma, thermal and chemical burns, toxic effects, and allergic reactions. Accurate diagnosis and identification of the underlying causative factor are essential for selecting appropriate therapeutic measures and preventing complications. The growing demand for aesthetic and durable restorations underscores the importance of continuous evaluation and improvement of material biocompatibility in contemporary dental practice.