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AFMN Biomedicine is an international, peer-reviewed, open-access journal dedicated to publishing high-quality original research and review articles in basic, translational, and clinical biomedicine, emphasizing discoveries of broad significance for the health sciences.

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Vol. 43, No. 1 (2026):

Published: 15.03.2026.

AFMN BIOMEDICINE

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Artificial intelligence (AI) represents a global phenomenon changing all spheres of human life. Biomedical engineering is no exception, as many AI systems are applied to biomedical engineering inventions. The European Union has enacted the new EU AI Act, one of the world’s first laws on AI. The main topic of this research is to examine what changes this new regulation brings to AI development in the field of biomedical engineering. An AI system applied in biomedical engineering is often considered a high-risk AI system, which means that AI developers are bound by a set of requirements and obligations to achieve a trustworthy, human-centric AI system. The authors analyze the impact and appropriateness of these requirements for developing AI systems in biomedical engineering using the legal dogmatic method, as well as by analyzing the secondary sources in the literature. The authors aim to present the current situation in AI regulation and make suggestions for further development.

Original article Online first
TRANSITION CHALLENGE: THE ROUGH ROAD TO ADULTHOOD IN JUVENILE IDIOPATHIC ARTHRITIS—EXPERIENCE FROM TWO CENTERS

By Dragana Lazarević, Hristina Stamenković, Stefan Đorđević, Dušica Novaković, Maja Zečević, Gordana Sušić

The transition process to adulthood is a challenging but crucial period for the future well-being of patients with chronic diseases. The objective of this study was to evaluate whether specific clinical variables and disease activity status are associated with improved transition readiness among patients with juvenile idiopathic arthritis (JIA) and their parents. JIA patients aged 12–18 years were enrolled in this cross-sectional study. Patient characteristics and laboratory data were collected, and disease activity was assessed using the Juvenile Arthritis Disease Activity Score (JADAS-10 and JADAS-27). All patients and their parents completed the Serbian version of the Transition Readiness Assessment Questionnaire (TRAQ) simultaneously. A total of 91 JIA patients (including 27 males and 64 females; median age 15.32 years, range 11.58–18 years), along with their respective parents, were enrolled in the study. Our results demonstrated that increased patient age and the use of biologic therapy, particularly etanercept, were significantly associated with higher TRAQ scores and improved transition readiness in both JIA patients (p < 0.001; p = 0.038) and their parents (p < 0.001; p = 0.035). Patient and parent TRAQ scores showed a strong positive correlation (ρ = 0.676, p < 0.001). No significant associations were found between other clinical variables (gender, JIA disease subtype, disease duration, disease activity status, extraarticular comorbidities, and autoimmune diseases in the patient’s family) and transition readiness. Older patient age and the use of biologic therapy, particularly etanercept, are positively associated with transition readiness in both JIA patients and their parents.

SGLT-2 inhibitors have been associated with increases in hemoglobin and hematocrit levels; however, the independence of this effect from confounding factors remains unclear in real-world settings. This study aimed to determine whether hemoglobin and hematocrit levels differed between patients with type 2 diabetes mellitus using SGLT-2 inhibitors and the control group and to investigate whether this effect was independent of factors such as age, sex, ferritin level, glycemic control, and renal function. Data were retrospectively collected from 205 adult patients at the Internal Medicine Clinic of Düziçi State Hospital. Patients were divided into two groups: those using an SGLT-2 inhibitor for at least three months (n = 103) and the control group (n = 102). Hemoglobin, hematocrit, ferritin, HbA1c, and eGFR levels were compared. Subgroup analyses and propensity score matching (PSM) were performed. Median hemoglobin (14.90 (12.6–18.2) vs. 13.00 (12.0–16.06) g/dL, p < 0.001) and hematocrit (45.20 (37.6–54.1) vs. 40.00 (32.2–49.6)%, p < 0.001) were significantly higher in the SGLT-2 group. This increase remained significant across all subgroups (sex, age, ferritin, HbA1c) and persisted after PSM (p < 0.001). Higher hematological parameters were observed with a treatment duration of ≥12 months. SGLT-2 inhibitors are associated with significantly higher hemoglobin and hematocrit levels in patients with type 2 diabetes, independent of iron stores and glycemic control. The underlying mechanism likely involves increased erythropoiesis beyond hemoconcentration. Periodic hematological monitoring is recommended. 

Original article Online first
RISK FACTORS FOR SPINAL ANESTHESIA – INDUCED HYPOTENSION DURING THE ELECTIVE CESAREAN SECTION

By Selena Djurić, Arsen Uvelin, Nikola Gvozdanović

Original article Online first
VACCINE HESITANCY IN PARENTS OF CHILDREN WITH CONGENITAL UROGENITAL ANOMALIES WITH HYPOSPADIAS AND UNDESCENDED TESTIS

By Mehmet Semih Demirtas, Sıddıka Songul Yalcin, Mustafa Tusat, Ismail Ozmen, Dilek Basar, Ahmet Salih Calapoglu

Original article Online first
COMPREHENSIVE MAXILLARY ANTERIOR TEETH AESTHETICS ANALYSIS WITH DEEP LEARNING IN DENTAL ANTHROPOMETRY

By Milena Kostić, Miloš Milovančević, Nadica Đorđević, Aleksandra Ilić, Marko Igić, Marija Đorđević, Nikola Gligorijević, Jana Pešić-Stanković, Goran Jovanović

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