Dynamics Of Differentiation Of Chromogranin A-Immunoreactive Endocrine Cells And Myenteric Plexus Of The Human Fetal Duodenum In The Third And The Fifth Month Of Development
The aim of the paper was to examine the neuroendocrine differentiation of endocrine cells and neurons of myenteric plexus of fetal duodenum. The material consisted of duodenums of 14 fetuses of different gender, in the third and fifth month of gestational age. Fetal duodenal tissue was fixed in the Bouin solution, routinely processed and embedded in paraffin blocks, from which were made the sections stained with HE and PAP immunostaining method for identification of chromogranin A (Cg A), marker of neuroendocrine differentiation. The number of endocrine cells was quantified by determining the numerical areal density, while the morphological development of myenteric plexus was determined by measuring its area. There is a large number of Cg A-immunoreactive endocrine cells in the mucosis of duodenum in the third and fifth month of development. These cells show a high degree of polymorphism in shape, size and immunoreactivity. No significant difference was observed in the number of cells in the epithelium of duodenum in the third and the fifth month of development. At the same time, myenteric plexus was significantly more developed in the duodenum in the fifth month of fetal development compared to the third month. The results indicate that the differentiation of Cg A-immunoreactive cells occurs earlier in the epithelium of the duodenum than in neurons of myenteric plexus.
Larsson L, Jorgensen LM. Ultrastructural and Cyto chemical Studies on the Cytodifferentiation of Duo denal Endocrine Cells. Cell Tiss Res. 1978;194(79).
2.
Grube D, Aunis D, Bader F, Cetin Y, Jorns A, Yoshie S. Chromogranin A (CgA) in gastro-entero pancreatic (GEP) endocreine system (CgA in the mammalian endocrine pancreas. Histochemistry. 1986;85:441–52.
3.
Cetin Y, Muller-Koppel L, Aunis D, Bader MF, Grube D. Chromogranin A (CgA) in the gastro-entero-pan creatic (GEP) endocrine system. Histochemistry. 1989;92:265–75.
4.
Ahlman H, Nilsson O. The gut as the largest endo crine organ in the body. Ann Oncol. 2001;12(63):68.
5.
Dayal Y. Neuroendocreine cells of the gastrointesti nal tract: Introduction and historical perspective. 1991.
6.
Moxey PC, Trier JS. Endocrine Cells in the Human Fetal Small Intestine. Cell Tiss Res. 1977;183:33 50.
7.
Fekete É, Bagyánszki M, Resch AB. Prenatal deve lopment of the myenteric plexus in the human fetal small intestine. Acta Biologica Szegediensis. 2000;44:3–19.
8.
Djuknić-Pejović M, Katić V, Petrović S, Nikolić I, Rančić G, Avramović V, et al. Histološke i histohemijske karakteristike mucina u tankom crevu humanog fetusa. Acta Fac Med Naiss. 2000;10:143–6.
9.
Nikolić I, Rančić G, Radenković G, Lačković V, V T, Mitić D, et al. Embriologija čove ka. 2007.
10.
Lebenthal E. Concept in gastrointestinal develop ment. In: Human gastrointestinal development. 1989.
11.
Bagyánszki M. Embryonic development of the hu man enteric nervous system and the enteric micro environment.Acta Biologica Szegediensis. 2002;46(12).
12.
Puri P, Rolle U. Development of the Enteric Nervous System.
13.
H YM. Functional development of the enteric nervous system - from migration to motility. Neuro gastroenterolog & Motility. 2008;20:20–31.
14.
Young MH, Hearn JC, Newgreen FD. Embriology and development of the enteric nervous system. Gut. 2000;47:12–4.
15.
Facer P, Bishop AE, Cole GA, Aitchinson M, Kendall CH, Aswegen G, et al. Developmental Preofile of Chro mogranin, Hormonal Peptides, and 5 - Hydroxytryp tamine in Gastrointestinal Endocrine Cells. Gastro enterology. 1989;97:48–57.
16.
Dubois PM, Paulin C. Gastrointestinal Somatostatin Cells in the Human Fetus. Cell Tiss Res. 1976;166:179–84.
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