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Investigation of possible effects of Exendin-4 during exposure to mild chronic stress on dehydroepiandrosterone-induced polycystic ovary syndrome in rats

By
Burcu Köksal ,
Burcu Köksal
Sedat Yildiz ,
Sedat Yildiz
Tugba Ozgöcer ,
Tugba Ozgöcer
Azibe Yildiz ,
Azibe Yildiz
Nigar Vardi ,
Nigar Vardi
Özlem Barutcu
Özlem Barutcu

Abstract

The purpose of the study was to investigate the effects of Exendin-4 on polycystic ovary syndrome (PCOS) in rats in chronic mild stress medium. For establishing the PCOS model, dehydroepiandrosterone (DHEA) (6mg/100g) in 0.2ml sesame oil was injected subcutaneously to 21-day old rats (n = 67). In addition, 0.2ml sesame oil was injected subcutaneously to the rats in groups involving solution injection only. At the initial stage of the study, the rats were grouped as control, solution and PCOS, whereas stress and Exendin-4 groups were also added in the second stage of the study. In PCOS groups, Exendin-4 was applied intraperitoneally (10μg/kg/day) in mild chronic stress medium for four weeks. The results revealed that weight, fasting blood glucose, fasting blood insulin and HOMA-IR levels in the rats with PCOS were significantly higher than in the other groups; also, corticosterone levels of stress groups were significantly higher than in the other groups. In addition, harmful effects of PCOS on ovarian tissues were observed in histological examinations. However, after Exendin-4 application in PCOS groups, weight, fasting blood glucose, fasting blood insulin, HOMA-IR and LH/FSH levels were decreased, whereas Exendin-4 application in PCOS group treated with stress was not as effective as the application of Exendin-4 on rats with PCOS. Exendin-4 application also increased the number of healthy follicles in PCOS group, whereas there was no change in the number of healthy follicles in PCOS+Stress group.

 

References

1.
Marin MT, Cruz FC, Planeta CS. Chronic restraint or variable stresses differently affect the behavior, corticosterone secretion and body weight in rats. Physiology & Behavior. 2007;90(1):29–35.
2.
Smith C. Using Rodent Models to Simulate Stress of Physiologically Relevant Severity: When, Why and How. Glucocorticoids - New Recognition of Our Familiar Friend.
3.
McDougall SJ, Paull JRA, Widdop RE, Lawrence AJ. Restraint Stress. Hypertension. 2000;35(1):126–9.
4.
Sun L, Ji C, Jin L, Bi Y, Feng W, Li P, et al. Effects of Exenatide on Metabolie Changes, Sexual Hormones, Inflammatory Cytokines, Adipokines, and Weight Change in a DHEA-Treated Rat Model. Reproductive Sciences. 2016;23(9):1242–9.
5.
Conway G, Dewailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Franks S, Gambineri A, et al. The polycystic ovary syndrome: a position statement from the European Society of Endocrinology. European Journal of Endocrinology. 2014;171(4):P1–29.

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Kannan Sridharan, Gowri Sivaramakrishnan

(2025)

Expanding therapeutic horizons: glucagon-like peptide-1 receptor agonists and sodium glucose transporter-2 inhibitors in poly cystic ovarian syndrome: a comprehensive review including systematic review and network meta-analysis of randomized clinical trials

Diabetology & Metabolic Syndrome, 17(1)

10.1186/s13098-025-01730-8

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